Gene description for Slc12a2
Gene name solute carrier family 12, member 2
Gene symbol Slc12a2
Other names/aliases 9330166H04Rik
Nkcc1
mBSC2
sy-ns
Species Mus musculus
 Experiment description of studies that identified Slc12a2 in Melanoma cells

1
Experiment ID1167
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample nameB16F1 (16.5 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
EV-TRACK -

2
Experiment ID1168
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample name B16F10 (16.5 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
EV-TRACK -

3
Experiment ID1169
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample nameB16F1 (100 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
EV-TRACK -

4
Experiment ID1170
Identified moleculeprotein
Extracellular vesicle typeExtracellular vesicles
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29907695   
OrganismMus musculus
Experiment descriptionCancer cells copy migratory behavior and exchange signaling networks via extracellular vesicles.
AuthorsSteenbeek SC, Pham TV, de Ligt J, Zomer A, Knol JC, Piersma SR, Schelfhorst T, Huisjes R, Schiffelers RM, Cuppen E, Jimenez CR, van Rheenen J.
Journal name EMBO J.
Publication year2018
SampleMelanoma cells
Sample name B16F10 (100 K)
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
miRNA
Methods used in the studyMass spectrometry
RNA sequencing
EV-TRACK -

5
Experiment ID3205
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34957415   
OrganismMus musculus
Experiment descriptionMelanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
AuthorsGarcía-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H.
Journal name Nat Cancer
Publication year2021
SampleMelanoma cells
Sample nameB16-F1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
EV-TRACK -

6
Experiment ID3206
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34957415   
OrganismMus musculus
Experiment descriptionMelanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
AuthorsGarcía-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H.
Journal name Nat Cancer
Publication year2021
SampleMelanoma cells
Sample nameB16-F10
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
Flow cytometry
EV-TRACK -

7
Experiment ID3207
Identified moleculeprotein
Extracellular vesicle typeSmall extracellular vesicles
MISEVSmall EVs
Identification method Mass spectrometry
PubMed ID 34957415   
OrganismMus musculus
Experiment descriptionMelanoma-derived small extracellular vesicles induce lymphangiogenesis and metastasis through an NGFR-dependent mechanism
AuthorsGarcía-Silva S, Benito-Martín A, Nogués L, Hernández-Barranco A, Mazariegos MS, Santos V, Hergueta-Redondo M, Ximénez-Embún P, Kataru RP, Lopez AA, Merino C, Sánchez-Redondo S, Graña-Castro O, Matei I, Nicolás-Avila JÁ, Torres-Ruiz R, Rodríguez-Perales S, Martínez L, Pérez-Martínez M, Mata G, Szumera-Ciećkiewicz A, Kalinowska I, Saltari A, Martínez-Gómez JM, Hogan SA, Saragovi HU, Ortega S, Garcia-Martin C, Boskovic J, Levesque MP, Rutkowski P, Hidalgo A, Muñoz J, Megías D, Mehrara BJ, Lyden D, Peinado H.
Journal name Nat Cancer
Publication year2021
SampleMelanoma cells
Sample nameB16-F1R2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectrometry
Flow cytometry
EV-TRACK -

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